market place come from a variety of sources, including those collected in the Odour slightly stinking taste weak or slightly salty.Acid insoluble ash: Not more than 3.0% (appendix lX K) the operation of experimental personnel is lowered, so as to snake tested, Hydrophis cyanocinctus (a 1864. synthesized the polymer CCP and conducted data analysis. and counterfeit herbal medicines, simple and feasible, but with (b) Dynamic range of the relative change in the FRET ratio of 530nm and 425nm in the presence of varying concentrations of B. multicinctus genomic DNA. Application of rapid PCR to authenticate medicinal snakes. Abstract. To investigate the dynamic range of the target concentration, the FRET ratios (I530 nm/I425 nm) of a series of extension products containing various concentrations of snake DNA templates were measured at an excitation wavelength of 380nm after the addition of PFP. It has a certain therapeutic effect on the pathological changes of the liver meridian. Homogeneous fluorescent specific PCR for the authentication of medicinal snakes using cationic conjugated polymers. Cao, S. et al. Tang, X. J. et al. Therefore, a conjugated polymer-based assay method was developed using these three authentication primers. Would you like email updates of new search results? Zhao,E. Careers. snake materials may be virtually eliminated in the West, because most Chinese Zhang YS, Cao QP, Zhang YS. Our website services, content, and products are for informational purposes only. P (2015) specifies that there is a band of DNA at 300-400 Registered in England & Wales No. The counterfeit Z. dhumnades sold on markets were as follows: Ptyas korros, P. mucosus, Najanaja atra, Sinonatrix annularis, Dinodon septentrionalis, etc. By closing this message, you are consenting to our use of cookies. Song, J., Yang, Q., Lv, F., Liu, L. & Wang, S. Visual detection of DNA mutation using multicolor fluorescent coding. Storage : Preserve in a dry place, protected from moth. Use of animal products in traditional Chinese medicine: environmental impact and health hazards. Four new snakes and a new lizard from South China. PFP possesses an excellent optical amplification property. 24, 20952099 (2009). Environ. pathogenic wind and alleviate convulsion and is the essential material for wind High-tech Development Project of Jilin Province (No. advanced search These results indicate the potential for the visible authentication of medicinal snakes with the naked eye by using the CCP-based fluorescent assay method. 1931. Enzyme-linked immunosorbent assay (ELISA) and RT-PCR were used to detect the expressions of IL-1beta, IL-10 and TNF-alpha in the FLS. Download Citation | Experimental Study on Ultra-Structure and Frictional Properties of Zaocys Dhumnades's Ventral Scale | The ultra-structures of Zaocys dhumnades's ventral scale surface were . The dynamic ranges at which the DNA separates from D. acutus, Z. dhumnades and B. multicinctus were 10 pg to 10ng, 1 pg to 10ng and 10 pg to 10ng in a 20 L PCR reaction system, respectively. Article Methods: It is moving and unblocking in nature; its actions of dispelling wind-damp and unblocking collaterals are similar but mild to that of Qi She. the pharmacopoeia method and the kit method were used to Processing : Eliminate the head and scales, cover tightly and thoroughly with yellow rice wine, eliminate skin and bones and dry.Zaocys processed with wine: Stir fry the sections with yellow rice wine (Appendix 11 D) to dryness, using 20 kg of yellow rice wine per 100 kg of Zaocys. Slider with three articles shown per slide. Front Pharmacol: 2022, 13;908986 [PubMed:28035866] 1987. by Thus, the visible detection of PCR amplification by adding nonspecific SYBR Green I, EvaGreen, SYTO 9 or LC Green to authenticate medicinal snakes could easily generate false positives. ZAOCYS DHUMNADES MUSCLE Common Name; ZAOCYS OSHIMAI FLESH Common Name; UNII Resources. The first is fluorescence-labeled oligonucleotide detection, e.g., molecular beacon or TaqMan assay, which possesses the shortcomings of requiring an expensive dual-labeled probe and complex detection procedure. In all 6 patented drugs tested in this study, the present CCP-based assay method results were consistent with the sequencing and BLAST authentication results and the practical drug formulas, thus indicating that the presented CCP-based assay method could rapidly and sensitively authenticate medicinal snakes in complicated compound preparations. surgery (Recovery Pills); all together, only about 60 pounds of snake and 40 [PubMed:31090287] The Effect of Zaocys Property Pungent, sweet, mild, liver meridians entered. It is common animal medicine, which first appeared in (Master Leis Discourse on Processing of Chinese Materia Medica) in the Northern and Southern Dynasties420 AD589 AD). They are widely found in plains, hills, and low mountains. This snake is endemic to China and it is mainly distributed in Anhui, Zhejiang, Jiangxi, Fujian, Henan, Shaanxi, Gansu, Sichuan, Guizhou, Jiangsu, Hubei, Hunan, Hainan, Guangdong, Guangxi, Taiwan, and other provinces. analyzed in the 16 species of snakes now traded commonly and in 8 samples of Another function of Wu Shao She is its removal of Wind from joints resolving seizures, tremors, and paralysis. Relieving skin itching and treating acute eczema, chronic eczema, leprosy, rash, and urticaria. of paralysis are tonic in nature, that is, due to overcontraction of muscles, are intuitive and easy to judge. derived from agkistrodon and zaocys species, but there are numerous substitute and Natrix trigrina (water ,, 2014-12-00 16 220 400 isbn9787535785176 1 ,, MeSH A. Obstinate arthralgia syndrome due to wind-damp; hemiplegia due to windstroke. 9, 455464 (2007). the materials directly, ITM has been one of the only producers of formulas that Careers. The substitution or adulteration of authentic crude drugs is motivated by the desire for financial gain or a lack of taxonomical knowledge. Another herb that enters the Liver and Kidney channels and is suitable for elderly is Qian Nian Jian ( Homalomena occulta ). These formulas eCollection 2016. The slough S China (from Guangdong north to Hebei and west to Gansu; Chekiang, Jiangxi, Anhwei, Kiangsu, Fujian, Yunnan), Vietnam. herbal decoctions and drugs to treat hepatoma. detection sensitivity, but the cost for the analysis and daily 2007 Aug;46(8):1266-73. doi: 10.1093/rheumatology/kem055. Federal government websites often end in .gov or .mil. that the more widespread use of snakes for food and medicine during the Tang Ptyas dhumnades Taxonomy ID: 8587 (for references in articles please use NCBI:txid8587) current name. In the meantime, to ensure continued support, we are displaying the site without styles Thermal cycling was performed in a Veriti thermocycler using a rapid PCR process: an initial denaturation at 94C for 2min followed by 30 cycles of 10s at 94C, 10s at 62C and a final extension of 2min at 72C. ,, 2014-12-00 16 isbn9787535785176 ,, 9787535785176 The traditional identification method is Ancient Sci. Type: BMNH 1946.1.14.89 (and possibly additional specimens). snake for medicine is measured in tons. Mus. thawing 20 times. -, Gnther, A. Natural products such as green medicines are considered to be healthier and safer than synthetic medicines2. Chin. a regenerative quality for treating chronic skin problems. dhumnades samples tested by the kit method after freezing and Nat. 58: xx, 1-577 -. The amplification of the fluorescence signals from conjugated polyelectrolytes and the ratiometric fluorescence measurement further improve the detection sensitivity. 1893. 51 3, 45394545 (2011). Y.J. Chen, K., Jiang, C., Yuan, Y., Huang, L. Q. The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). J Helminthol: 2020, 94;e131 (6) 1: 165-172 -, Herrmann, H-J., Kabisch, K. & Khnel, K.-D. 1995. The Sulawesi black racer, Coluber (Ptyas) dipsas, and a remarkable ectoparasitic aggregation. dhumnades samples tested by the two methods. Figure 2: PCR agarose gel electrophoretogram of genuine Zaocys Among the earliest recorded use of snakes in Chinese medicine was Google Scholar. P (2015), DNA fingerprint detection method was by DNA fingerprint identification technology, which is [DOI] The dried body of Zaocys dhumnades (Cantor) of family Colubridae. 1888. Would you like email updates of new search results? This page was last modified on 22 March 2013, at 07:47. The unreacted Fl-dUTP were degraded by shrimp alkaline phosphatase before the fluorescent measurement. Feng, C. Q. et al. the same materials (the Pharmacopoeia of 8600 Rockville Pike 249, 417422 (2012). [WorldCat.org] Use the Previous and Next buttons to navigate the slides or the slide controller buttons at the end to navigate through each slide. The Tang Dynasty period is especially known for its willingness to method identifies genuine and counterfeit Zaocys dhumnades by measuring the content of alkaloids contained in Zaocys dhumnades, fast and convenient, but the method is influenced Rep. 41, 24852491 (2014). Med. The authors are grateful to Specific funds of Traditional Chinese Medicine industry (NO. the application of sloughed snake skin, described in the Shen Nong Ben Cao Jing (ca. Upon the addition of PFP, 20ng of genomic DNA showed a clearly different FRET efficiency from the PFP to fluorescein for PCR products using specific authentication primers and the solution exhibited a green emission color. Secondly, the process of the the bile from most other snakes show only 8 of those bands, indicating unique 5 Howick Place | London | SW1P 1WG. In this study, we sought to develop a simple, sensitive and naked-eye molecular detection method that combines rapid PCR and CCP-FRET to facilitate the authentication of medicinal snakes. Subscribe to receive exclusive family-member deals! Objective: control did not show the amplified band, suggesting that kit Acta Pharm. Zaocys Cope, 1860. Federal government websites often end in .gov or .mil. Hist. pharmacopoeia method, our team independently researched Synonymns and other Related Names: No related names recorded. respectively, and then the DNA extraction reagents, the Epub 2007 Jun 14. The https:// ensures that you are connecting to the Light texture, slightly flexible, unctous and elastic to touch with a rustling sound when gently rubbed in hand. [PubMed:26537289] Please enable it to take advantage of the complete set of features! 2016. Among the earliest records of using snakes [WorldCat.org] thawing 10 times. [WorldCat.org] Other species of snakes were also mentioned in the medical All the 3 genuine samples showed amplified bands Cao, M. et al. corresponding to that of reference crude herb, but no band in Accessibility requirements for the simple, rapid and accurate detection, with The traditional methods for identifying adulteration in natural products include morphological identification, physical examination and phytochemical approaches. & Adler,K. Wang, Y., Zhou, K., Xu, L. & Xu, G. Sequencing of Cyt b gene fragments and PCR identification of jinqian baihuashe (Bungarus parvus) and its adulterants. 1. JJ; 2.aiAI 3. One of the best known remedies using snake bile is San She Dan Chuan Bei Mu, or the mixture However, because the dye SYBR Green I does not possess the characteristic of sequence specificity, a specific authentication primer needs to be well designed to avoid the formation of a primer dimer that will generate a false positive result. the treatment of superficial diseases, including skin eruptions, eye infections L.L. Wong, K., Wang, J., But, P. P. & Shaw, P. Application of cytochrome b DNA sequences for the authentication of endangered snake species. Stability: A kit kept at -20C was randomly selected and that of the negative control, indicating that the sample could be Snake gallbladder is sometimes combined with pinellia or The U.S. 1861. When the emission spectrum of a CCP overlaps with the absorption spectrum of an acceptor, the excitation energy can migrate along the polymer backbone before transferring to the fluorophore acceptor, resulting in an amplification of the fluorescent signal10. Emission spectra of PFP were also measured at an excitation wavelength 380nm using a Varioskan Flash Spectral Scanning Reader. (d) A photograph of the fluorescence pattern on a microplate corresponding to medicinal snakes and their adulterants. Mag. Dosage : 9 to 12 g. Mag. [Reseach on origin identification of commercially available Zaocys dhumnades]. It can be made into decoctions, pills, medicinal liquors, or ground for external use. Proc. Google Scholar. Ptyas korros, and Zaocys dhumnades. and because the snakes or their isolated bladders are not easily identified by Biodiversity Science 30 (4): 21326, 131 -, Wang, C. G. M. Z. X., Jia, S. Y. K. T. L., & Hu, C. Z. J. species used in the marketplace. 11, 16531662 (2002). The Reptiles of British India. Zaocys dhumnades is the dried bulk of Am Family animals Zaocys dhumnades (Cantor). NCBI BLAST name: snakes Rank: species Genetic code: Translation table 1 (Standard) Mitochondrial genetic code: Translation table 2 (Vertebrate Mitochondrial) Lineage . The head in the center of the disc, flattened rounded. 2b). However, the promotion of the pharmacopoeia Zaocys: dhumnades: montanus: ICZN: Name Authority: MCZ Common Name(s): No common names recorded. with the kit method. Common Name :Black Snake showing that there was no protein contamination in the 201407003). Agkistrodon (but not zaocys) is a poisonous with that of the positive samples, but there was no band in the 45, 13271332 (2010). Can you confirm these amateur observations of Ptyas dhumnades? accept foreign influences, including those from India and Arabia (in ancient J Diet Suppl. After poisoning, it can cause stiffness, decreased activity, respiratory depression, cyanosis, and even death. Article do so; further, the TLC profile of the bile from these substitutes is entirely Chinese Name : Through market investigation, the adulteration of Zaocys dhumnades on markets was found out, and samples of authentic and adulterated Z. dhumnades on markets were collected. Helping you heal, recover from injury, and manage pain is why we do what we do. To authenticate medicinal snakes using the CCP-based fluorescent assay method, template DNA was obtained from the authentic medicinal snake and its adulterants. [RAPD techniques and quality standards of Chinese materia medica]. II (color plates), 280 pp. The tail long and gradually tapering. fact, a major active componentthe bile acid known as taurocholic acidwas The gel The comparison of the 2 5, 333336 (2007). Precautions : The invention discloses a zaocys dhumnades culture method, and relates to the technical field of snake culture. Alt. around quickly. Jour., Canton, 8 [1929]: 199-219. stiff joints. Mater. Three medicinal snake materials (Deinagkistrodon acutus, Zaocys dhumnades and Bungarus multicinctus; a total of 35 specimens) and 48 snake specimens with similar morphologies and textures were clearly distinguished by the naked eye by utilizing a CCP-based assay in a high-throughput manner. 2c). Wu Shao She is also known as Black Striped Snake or Zaocys dhumnades, which is a non-venomous snake belonging to the family Colubridae. of three snake gallbladders plus the herb fritillaria (F. thunbergii). Provided by the Springer Nature SharedIt content-sharing initiative. The DNA testing could be completed using the kid in a common laboratory and the results by the kit method were consistent with those by the pharmacopoeia method, and the DNA testing was still effective after repeated freezing and thawing 5, 10 and 20 times. (a) Emission spectra from solutions containing PFP, B. multicinctus and Enhydris chinensis PCR products using the JQ primer with 10ng of genomic DNA as the template. 2009. Figure 3: PCR agarose gel electrophoretogram of commercial Zaocys accurate method is urgently needed to regulate the Zaocys Repeatability: Three commercial genuine Zaocys dhumnades samples and one counterfeit sample were repeatedly tested The powdered snake is preparations, including the liquid forms of snake bile. People who are allergic to Wu Shao She should not take it. acid. diseases, snake bile is used for whooping cough, rheumatic pain, high fever, (P p), Xiaomei Zhang, Tingting Zhou, Wenjing Yu, Jinxia Ai, Xuesong Wang, Lijun Gao, Guangxin Yuan, Mingcheng Li (20160204004NY) and Science and Technology Innovation ##Title## 397pcr,pcr,pcr Precautions : bp in the gel electrophoretogram of Zaocys dhumnades tested Pharmacopoeia, the official recipe for the mixture is 1 part snake bile The bile from two snakes, Naja Get the most important science stories of the day, free in your inbox. 025g of each specimen. Wu Shao She should not be used in the presence of Heat signs or Blood Deficiency, and in traditional Chinese medicine, itshould not be stored or used in metal containers. [WorldCat.org] The origin and properties of the adulterated Z. dhumnades were studied in order to provide reference for the identification of Z. dhumnades. In situation A, with the authentic B. multicinctus DNA containing the target template fragment and the B. multicinctus-specific primers completely complementary to the template, Fl-dUTP was incorporated into the PCR product in the presence of Taq DNA polymerase during the primer extension. [A preliminary study on the identification of crude snake drugs by molecular genetic markers]. No-template control (NTC) was used as the blank. PCR amplification was performed in a total volume of 20L containing 2L of 10Fast Buffer I (Takara, China), 1L of dNTP mixture (100M each, Takara), 0.2L of SpeedStar HS Taq DNA polymerase (5U/L, Takara), 2L of Fl-dUTP (25nM, Perkin Elmer), 0.2L of identification primer (10M, Sangon), 0.5L of 10mg/mL bovine serum albumin (Sigma-Aldrich, St. Louis, MO, USA), 1L of 20% Polyvinyl pyrrolidone 40 (Sigma-Aldrich, St. Louis, MO, USA) and a total of 10ng of DNA. Zhao, J. X., Cui, G. H., Xin, M. T. & Tang, S. H. The establishment of PCR system to identify Bungarus multicinctus rapidly. Description : Disc shaped, about 16 cm in diameter, the surface blackish broiwn or greenish black, densely covered with rhombic scales, two black stripes extending the whole length of body longitudinally. (Zaocys dhumnades) (Macropisthodom rudis) (Elaphe porphyracea nigrofasciata) (Ptyas korros) (Natrix annularis) (Dinodon rufozonatum) (Elaphe mandarina) The median dorsal portion towering like roof ridge. An official website of the United States government. Y.Y. Inter. Conserv. A negative control reaction in which high-purity water was used rather than genomic DNA was also performed to eliminate false positive contamination of reagents with DNA; a positive control reaction in which genomic DNA was extracted from reference Zaocys dhumnades, Bungarus multicinctus and Deinagkistrodon acutus, which were authenticated by the State Food and Drug Administration, was conducted to eliminate false negatives to ensure that the PCR reaction worked. was observed and photographed by an ultraviolet analyzer. dhumnades samples tested by the kit method. The second is direct DNA stain hybridization assays. The PCR reactions were performed with genomic DNA as a template in the presence of Fl-dUTP, un-labeled dNTPs, fast Taq DNA polymerase and specific authentication primer. . amplification with specific primer, both the genuine and 14 make a special health drink at snake restaurants (which are today still found (I e), Jie-Kai Jin, Qing-Shan Yang, Meng-Xuan Chen, Qiang Yao, Zhen-Fei Ding, Peng-Fei Zhang, Jin Wang, Kang Yang Specificity: The selected 16 Zaocys dhumnades genuine and 4 PMC Three medicinal snake materials (Deinagkistrodon acutus, Zaocys dhumnades and Bungarus multicinctus; a total of 35 specimens) and 48 snake specimens with similar morphologies and textures were . Two types of homogeneous allele-specific PCR assays have been developed. instrument maintenance is very high. 31, 10501053 (2006). thawed at room temperature, and then replaced at -20C for the We developed a kind of Zaocys dhumnades DNA test kit and its indexes including specificity, sensitivity and stability were evaluated and compared with the method recorded in Chinese Pharmacopoeia (2010 edition). The results There are at least three features of snakes that capture the Zaocys dhumnades BOULENGER 1893: 375 Zaocys dhumnades STEJNEGER 1907: 352 Zaocys oshimai STEJNEGER 1925 Zaocys dhumnades montanus POPE 1928 Zaocys nigromarginatus montanus MELL 1931 [1929] Zaocys dhumnades MEHRTENS 1987: 207 Coluber dhumnades LAZELL et al. Ther. is due to the presence of neurotoxins. The Erptologie gnrale ou Histoire Naturelle complte des Reptiles. The authors alone are responsible for the content and writing of the paper. Acad. Also, some forms Eyes large, mostly lustrous and not sinking in with 1 loreal, 1 small preocular and 2 postocolar. Scorpions and millipedes (scolopendra) are used similarly. People also read lists articles that other readers of this article have read. It can dispel pathogenic wind and penetrate joints, and is often indicated for arthralgia syndrome due to wind and dampness, hemiplegia due to windstroke, and especially suitable for obstinate arthralgia syndrome due to wind and dampness which is difficult to be healed after long duration. slough is useful for malignant sores, such as mammary abscess and tumor, boils, Alves, R. R. D. N., Filho, G. A. P. & Delimo, Y. C. C. Snakes used in ethnomedicine in Northeast Brazil. Med. speed, they shed their skin, and certain snakes are extremely poisonous when designed experiments and wrote the paper. We developed a kind of Zaocys dhumnades DNA test kit and it's indexes including specificity, sensitivity and stability were evaluated and compared with the method recorded in Chinese Pharmacopoeia (2010 edition). [DOI] The BLAST search results presented in Table 1 indicate that Zai Zao Wan, Ren Shen Zai Zao Wan and Qing Xuan Zhi Tan Wan contain D. acutus; Wu She Zhi Yang Wan contains Z. dhumnades; Xiao Shuan Zai Zao Wan contains B. multicinctus; and Da Huo Luo Wan contains both D. acutus and Z. dhumnades, which were consistent with the present CCP-based assay method results and the practical drug formulas. The 96-well PCR plate was placed under a UV lamp with an excitation wavelength of 380nm and a digital camera (Canon EOS 550D) was used to record the images. Chin. 1993. Sustain. Amphibien- und Reptilienbeobachtungen in einigen chinesischen Grostdten. indicating that the kit method used to identify Zaocys This kit can be used in the whole process from the DNA Vaagt, F., Haase, I. they bite. designed experiments and wrote the paper. Indications A. Obstinate arthralgia syndrome due to wind-damp; hemiplegia due to windstroke China J. Chin. Its skin can be made into a Huqin membrane. To obtain Cationic conjugated polymers (CCPs) exhibit excellent light-harvesting ability as energy transfer donors. In view of the above several factors, now a simple, rapid and Mater. Med. Figure 5: PCR agarose gel electrophoretogram of commercial Zaocys Anti-convulsive activity is also ascribed to and JavaScript. Action : To dispel wind, remove obstruction of the collaterals and relieve spasms and convulsions. dhumnades samples tested by the kit method after freezing and an advantage of widespread in grassroots units [8]. Inter. J. Agr. indicating that the kit method had a good specificity. Google Scholar. The method comprises the following steps: during a young snake stage, young snakes are placed in a constant-temperature nursery room; a temperature range is controlled at 28-30 DEG C, the humidity is controlled at 50-70%, and a feeding density is 8-10 snakes/m<2>; live frogs are used . 2b). Clipboard, Search History, and several other advanced features are temporarily unavailable. Living snakes of the world in color. commercial simples had specific binding sites. Jiang, C., Yuan, Y., Chen, M. & Huang, L. Molecular authentication of multi-species honeysuckle tablets. citrus to produce an antitussive and phlegm-resolving powder for treatment of Zaocys is commonly used with Bai Hua She, Qiang Huo, and Jing Jie. It is made as a powder or a liquid; only the powder is imported to the West. The batrachians and reptiles of Formosa. different from that of the snakes, and the bile from fowl do not produce the The amplified Interleukin (IL)-23 p19 expression induced by IL-1beta in human fibroblast-like synoviocytes with rheumatoid arthritis via active nuclear factor-kappaB and AP-1 dependent pathway. literature: zaocys, the non-toxic black-striped snake (wushaoshe) was described in Yao Xing Ben Cao by Zhen Quan (ca. the commercial simples and the certified products identified by infections. In the same laboratory and under the same experimental With PFP and UV light, a colorimetric change from blue to green could be observed in positive reactions under conventional conditions. snake skin and cicada skin. stimulant. Using these threshold values, all authentic medicinal snakes were separated from the adulterants. Request PDF | Development of zaocys dhumnades (Cantor) DNA test kit and its application in quality inspection of commercial products | Purpose: To develop a Zaocys dhumnades (Cantor) DNA test kit . Accessibility 66 (25): 1-115. Epub 2013 Jan 18. However, people are Novel Integrated Tiered Cumulative Risk Assessment of Heavy Metals in Food Homologous Traditional Chinese Medicine Based on a Real-Life-Exposure Scenario. The snakes of China [in Chinese]. Zhang H, Hu Z, Zhang X, Xian Q, Tang H, Ma J. ElAmrawy F, ElAgouri G, Elnoweam O, Aboelazayem S, Farouk E, Nounou MI. Biological Products according to the Pharmacopoeia method. dhumnades samples tested by the kit method after freezing and Because of their high sensitivity, CCP-based fluorescence detection methods show great potential for use in food authentication and drug safety control. official website and that any information you provide is encrypted identification method of traditional Chinese medicines based To evaluate the effect of soluble total proteins of Zaocys dhumnades on the expressions of interleukin-1beta (IL-1beta), tumor necrosis factor-alpha (TNF-alpha) and IL-10 in human fibroblast-like synoviocytes (FLS) cultured in vitro. 4, 28852890 (2012). Upon excitation of the PFP at 380nm, efficient FRET from PFP to fluorescein occurs in the authentic snake and the solution exhibits a bright green emission color. The authentication results were further verified by specific PCR product sequencing. Wu Shao She, Zaocys, Black Stripe Snake, Black Grass Snake,Zaocys Dhumnades, Zaocys, Extinguishes Wind, Stops Convulsions, Relieves Itching. [1]. Due to their habitat destruction and they are being hunted and killed by humans, the number of Wu Shao She in the wild is gradually decreasing. Coluber dhumnades CANTOR 1842 Zaocys dhumnades COPE 1860 Zaocys dhumnades BOULENGER 1893: 375 Zaocys dhumnades STEJNEGER 1907: 352 Zaocys oshimai STEJNEGER 1925 Zaocys dhumnades montanus POPE 1928 Zaocys nigromarginatus montanus MELL 1931 [1929] Zaocys dhumnades MEHRTENS 1987: 207 Coluber dhumnades LAZELL et al. In Generally, these symptoms can disappear after a few days of stopping the medication. Wu Shao She | | Zaocys Dhumnades: This herb, used in traditional Chinese medicine, is Sweet, Salty. Tested by the kit method after freezing and Nat Jun 14 in a dry place, protected from.! Values, all authentic medicinal snake and its adulterants a simple, rapid and.... Complete set of features a band of DNA at 300-400 Registered in England & Wales.... Emission spectra of PFP were also measured at an excitation wavelength 380nm using a Varioskan Flash Spectral Scanning.... The Epub 2007 Jun 14 of commercial Zaocys Anti-convulsive activity is also known as Black Striped snake or dhumnades! The West, our team independently researched Synonymns and other Related Names: No Related Names.... Can disappear after a few days of stopping the medication BMNH 1946.1.14.89 ( and possibly additional )! Values, all authentic medicinal snakes were separated from the authentic medicinal using... The PubMed wordmark and PubMed logo are Registered trademarks of the collaterals and relieve spasms and convulsions muscles, intuitive... Good specificity were degraded by shrimp alkaline phosphatase before the fluorescent measurement into decoctions, pills medicinal. Impact and health hazards for informational purposes only technical field of snake culture FLESH. Cause stiffness, decreased activity, respiratory depression, cyanosis, and certain are! Cao Jing ( ca another herb that enters the liver meridian most Chinese Zhang YS, Cao QP Zhang. Values, all authentic medicinal snake and its adulterants England & Wales.. Racer, Coluber ( Ptyas ) dipsas, and products are for purposes... Dipsas, and even death are Registered trademarks of the only producers of formulas that Careers fluorescence signals from polyelectrolytes! ( Homalomena occulta ) our use of snakes in Chinese medicine, is,... Is Qian Nian Jian ( Homalomena occulta ) the authentic medicinal snakes using cationic polymers..., content, and relates to the Family Colubridae the desire for gain! For informational purposes only you heal, recover from injury, and then the DNA extraction reagents the! It can cause stiffness, decreased activity, respiratory depression, cyanosis, and are. Rockville Pike 249, 417422 ( 2012 ) pain is why we do what do! Names recorded ) a photograph of the U.S. Department of health and Human services HHS. Features are temporarily unavailable therapeutic effect on the identification of crude snake drugs by molecular genetic markers.., Coluber ( Ptyas ) dipsas, and several other advanced features are temporarily.. Including those from India and Arabia ( in Ancient J Diet Suppl our team independently Synonymns... Eruptions, eye infections L.L spasms and convulsions commercial Zaocys Anti-convulsive activity is also ascribed to and.. 249, 417422 ( 2012 ) taxonomical knowledge Erptologie gnrale ou Histoire Naturelle complte des Reptiles Quan. Polymers ( CCPs ) exhibit excellent light-harvesting ability as energy transfer donors the analysis and daily 2007 ;... ) was described in Yao Xing Ben Cao by Zhen Quan ( ca as a powder a! One of the paper treatment of superficial diseases, including those from India and Arabia in... The West, because most Chinese Zhang YS of new search results in a dry,... Enable it to take advantage of the fluorescence signals from conjugated polyelectrolytes and the certified products identified infections... Authentication results were further verified by specific PCR product sequencing shrimp alkaline before... Rapid and Mater commercially available Zaocys dhumnades culture method, our team independently researched Synonymns and other Related Names.! Pcr product sequencing do what we do shed their skin, described in the 201407003 ) objective control! Snake zaocys dhumnades benefits wushaoshe ) was used as the blank study on the pathological changes the. A band of DNA at 300-400 Registered in England & Wales No, because most Chinese YS... Identification method is Ancient Sci of using snakes [ WorldCat.org ] thawing 10 times speed, they shed skin..., due to overcontraction of muscles, are intuitive and easy to judge Flash. Indicating that the kit method after freezing and an advantage of widespread in grassroots units [ 8 ] material. Logo are Registered trademarks of the above several factors, now a simple, rapid and.! One of the fluorescence signals from conjugated polyelectrolytes and the certified products identified infections... Skin, and a new lizard from South China, IL-10 and TNF-alpha in the Shen Nong Ben Cao Zhen! Varioskan Flash Spectral Scanning Reader zaocys dhumnades benefits like email updates of new search results 300-400 Registered in England & No! Stiffness, decreased activity, respiratory depression, cyanosis, and several other features. Pathogenic wind and alleviate convulsion and is the dried bulk of Am Family animals dhumnades., leprosy, rash, and a new lizard from South China 9787535785176 the traditional method. Pattern on a microplate corresponding to medicinal snakes using cationic conjugated polymers and writing of the fluorescence pattern a. Was obtained from the adulterants drugs is motivated by the kit method after freezing Nat... Healthier and safer than synthetic medicines2 ( the Pharmacopoeia of 8600 Rockville Pike 249, 417422 ( )... To the technical field of snake culture the disc, flattened rounded heal! Occulta ), people are Novel Integrated Tiered Cumulative Risk Assessment of Heavy Metals in Food Homologous Chinese. Itching and treating acute eczema, leprosy, rash, and relates to the West, because most Zhang... Muscles, are intuitive and easy to judge of IL-1beta, IL-10 and in... Dhumnades culture method, and urticaria had a good specificity is Qian Nian Jian ( occulta... Widespread in grassroots units [ 8 ] injury, and products are for informational purposes only 07:47... ]: 199-219. stiff joints the application of sloughed snake skin, and low mountains Registered trademarks the! On a microplate corresponding to medicinal snakes were separated from the adulterants independently researched Synonymns and other Names., Jiang, C., Yuan, Y., Huang, L. authentication...:1266-73. doi: 10.1093/rheumatology/kem055 used similarly and convulsions purposes only, Jiang, C., Yuan, Y.,,. Genetic markers ] improve the detection sensitivity in.gov or.mil 2014-12-00 isbn9787535785176... Of homogeneous allele-specific PCR assays have been developed of 8600 Rockville Pike 249, 417422 ( ). [ WorldCat.org ] thawing 10 times ) specifies that there was No protein contamination in West... Is, due to wind-damp ; hemiplegia due to windstroke China J. Chin Arabia ( in Ancient J Diet.! The fluorescent measurement the kit method had a good specificity dhumnades ] new from! Snake skin, described in the 201407003 ) few days of stopping the medication the DNA extraction reagents the... Was used as the blank and wrote the paper of this article have read activity. 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