sebastian kadener lab

de la Mata M., Alonso C.R., Kadener S., Fededa J.P., Blaustein M., Pelisch F., Cramer P., Bentley D. and Kornblihtt A.R. Biol., The University of Buenos Aires, Buenos Aires, Argentina, Ph.D., The University of Buenos Aires, Buenos Aires, Argentina, Undergraduate Departmental Representatives, Heller School for Social Policy and Management, Rabb School: Graduate Professional Studies, Graduate Professional Studies (Online Programs). Now the stage is set for the most exciting questions, which we are currently working in the lab, by combining molecular, computational and RNA biology with advances techniques in genetics and neurosciences. He received his BS in Biochemistry and Molecular Biology in 2022 from Dickinson College in Carlisle Pennsylvania. 502. In addition, we are extending our studies to additional tumor models. You can add this document to your study collection(s), You can add this document to your saved list. We believe that this is reminiscent of changes in ISC proliferation observed in animals that feed infrequently, such as the Burmese python, where ISC proliferation is triggered following a large meal as a result of gut distortion. Rosenstiel332781-736-4915skadener@brandeis.edu, Department of Biology Volen 206MS 008 Brandeis University 415 South Street Waltham, MA 02453. cabut during early embryogenesis, dorsal closure and nervous system development. Rabin A, Zaffagni M, Ashwal-Fluss R, Patop IL, Jajoo A, Shenzis S, Carmel L, Kadener S. Extensive tissue-specific expression variation and novel regulators underlying circadian behavior. What are the key functions of these molecules in the brain and during aging? Proximity labeling utilizes enzymes that generate reactive radicals to covalently tag neighboring proteins. Associate Professor, Brandeis University. From 2002 to 2008, Sebastian Kadener was a postdoc working in the Michael Rosbash laboratory. Antibodies allow protein visualization by immunostaining, biochemical study by immunoprecipitation and western blot, and proteomic study by IP-MS. A polar mechanism coordinates different regions of alternative splicing within a single gene. Now, in an article published in the prestigious journal Molecular Cell, the lab of Dr. Sebastian Kadener at the Hebrew University of Jerusalem, in collaboration with the lab of Prof.. Bartok O., Teesalu M., Pandey V., Hanan M., Poukkula M., Havula E., Moussaieff A., Vodala S., Nahmias Y., Kadener S.* and Hietakangas V.* Cabut-dependent repressive branch of the sugar sensing transcriptional network regulates glyceroneogenesis. * Corresponding authors. In the Bandopadhayay Lab, Jess is applying genomic approaches to optimize novel treatment approaches for pediatric brain tumors, and is also working to characterize the role long non-coding RNAs play in the formation of Diffuse Intrinsic Pontine Gliomas. PubMed ID: 31702556 Strikingly, these phenotypes are reminiscent of the pathophysiology of cystic fibrosis (CF), as in CF the non-functional CF transmembrane conductance regulator (CFTR) increases ENaC activity resulting in chronic dehydration of the intraluminal surface liquid in organs such as kidney, colon, lung and sweat glands. Aaron is an Attending Pediatric Neuro-Oncologist at the Dana-Farber/Boston Childrens Cancer and Blood Disorders Center. Coupling of transcription with alternative splicing: RNA pol II promoters modulate SF2/ASF and 9G8 effects on an exonic splicing enhancer. For example, we showed that circRNA biogenesis competes with canonical splicing, showing that circRNAs can function incisas RNA traps. Zaffagni M, Kadener S. NEAT1 is overexpressed in Parkinson's disease substantia nigra and confers drug-inducible neuroprotection from oxidative stress. We use Drosophila as a model system to characterize the mechanisms underlying communication between cells, tissues, and organs. Jess earned her PhD in Neuroscience working in the laboratory of Dr. Thomas Clandinin, utilizing the Drosophila visual system and forward genetics to study synapse development and axon degeneration. From a number of screens (receptome-wide RNAi screen, miRNA screens, etc. Regulation of RNA metabolism is emerging as a major regulatory hub for gene expression control in tissues like the brain and the muscle, which don't undergo cel. ( a) Simplified overview of three stages of the gypsy retrotransposon life cycle, (i) parent insertion in the genome, (ii) the transcribed RNA (genomic RNA), and (iii) the new copy inserted in the genome. In college, he minored in art history and loves spending hours at art museums. Recent reports revealed that circRNAs are expressed in developmental- and tissue-specific manners and are highly enriched in the nervous system, particularly in synapses. Fun fact: Leslie played basketball through college and is now an avid cyclist. Or do you know how to improve StudyLib UI? * Corresponding authors. Ph.D. University of So Paulo (USP)- Brazil. Eric joined the Bandopadhayay Lab in 2020 where he is working to characterize driver mutations found in pediatric brain tumors and developing new approaches to treat these children with targeted therapies. I am a PhD student in the lab since 2018 after graduating from The College of Wooster. There, she studied the oncogenic role of certain signaling molecules in the Hedgehog signaling pathway, and became extremely interested in advancing cancer therapy research. Sayan is a graduate student in the Molecular and Cell Biology program at Brandeis and he is investigating the role of circRNA in aging and neurodegeneration. Cold Spring Harbor Laboratory Online. I am a junior who is majoring in biochemistry. Lerner I. Studies from our lab and others have documented that many signaling pathways trigger stem cells to adjust their rate of proliferation/differentiation to meet the demand for tissue regeneration. Gut tumors can be induced in adult flies by overexpressing an active form of the transcriptional co-activator yorkie/YAP using the intestinal stem cell driver escargot-GAL4. Uncovering molecular and physiological functions of circRNAs: Circular RNAs (circRNAs) are highly abundant RNAs produced by circularization of specific exons. In addition and most importantly, genome-wide libraries of transgenic RNAi lines are available that allow knockdown of any Drosophila gene in an organ or tissue-specific manner. The EMBO journal 20 (20), 5759-5768, 2001. Further, our work and that of others has suggested functions of these moleculesin vivo. Weiss R., Bartok O., Mezan S., Malka Y and Kadener S. Synergistic Interactions between the Molecular and Neuronal Circadian Networks Drive Behavioral Circadian Rhythms in. 369. Fun fact:In her free time, Marissa loves to go on bike rides, do yoga and walk her dog. These tools, which are available viaDRSC/TRiP Functional Genomics Resources, include: DIOPTDIOPT-DISTGene2FunctionGLADBioLitMineUP-TORR, RSVPSnapDragonFlyPrimerBankFind CRISPRSNP-CRISPRDGET. This plasticity is the result of the existence of very efficient input pathways that convey the external signals into the core oscillator machinery. Our laboratory is interested for understanding how molecular processes in the brain determine behavior with special emphasis on RNA metabolism. Thisrobustnessis the result of multiple layers of regulation that extend beyond the single-cell level. Belacortu Y., Weiss R., Kadener S. and Paricio N. Transcriptional activity and nuclear localization of Cabut, the, Belancortu Y., Weiss R., Kadener S. and Paricio N. Expression of, Van der Linden A.M., Beverly M., Kadener S., Rodriguez J., Wasserman S., Rosbash M. and Sengupta P. Genome-Wide Analysis of Light and Temperature-Entrained Circadian Transcripts in, Fathallah-Shaykh H.M., Bona J.L. Understanding how circadian rhythms are generated, maintained, and adapted to changing conditions is key, as several diseases such as cancer and depression are associated with misalignment of the circadian clock with the environment. Circadian clocks are also exceptionallyplasticas they can quickly and specifically adjust to specific environmental cues. Cramer P., Srebrow A., Kadener S., Werbajh S., de la Mata M., Melen G., Nogues G. and Kornblihtt AR. One of these translated circRNAs is circMbl. Fun fact:Riley chose her English name when she moved to the U.S because it means courage. Alternative splicing regulates biogenesis of miRNAs located across exon-intron junctions. Ashwal-Fluss R., Meyer M., Pamudurti N.R., Ivanov A., Bartok O., Evantal N., Hanan M., Memczak S., Rajewsky N.* and Kadener S.* CircRNA biogenesis and canonical splicing compete with each other. For example, little is understood about how the simultaneous growth and differentiation of different tissues and organs are coordinated and how the development of different cell types and tissues is integrated within an organ. Fun fact: Like many Swedish people, Eric has completed Vasaloppet, a 60-mile cross-country skiing race (twice!). S Kadener, P Cramer, G Nogus, D Cazalla, M de la Mata, JP Fededa, . and Kornblihtt A.R. as achieved by mapping signal pathway activities in various cell types), and the physiological roles of various cells. Chen TC, Tallo-Parra M, Cao QM, Kadener S, Bttcher R, Prez-Vilar G, Boonchuen P, Somboonwiwat K, Dez J, Sarnow P. A lncRNA survey finds increases in neuroprotective LINC-PINT in Parkinson's disease substantia nigra. While at UMass Amherst, Marissa worked in the Facette Lab studyong asymmetric cell division in maize andArabidopsis thaliana. Followers. Selected for Faculty of 1000 (Factor 6 Must Read). The technologies we use for CRISPR screening in Drosophila cells are extensible to other cell types. Software. Kadener S., Cramer P., Nogues G., Cazalla D., de la Mata M., Fededa J., Werbajh S., Srebrow A. and Kornblihtt A. These studies have allowed us to better characterize what cell types are present in different gut regions and provide us with new powerful data sets with which we can better characterize the transcriptional networks that define lineage relationships among cell types, cell-cell interactions among cell types (i.e. Olivia received her B.A. In addition, skeletal muscles produce various myokines that influence metabolic homeostasis, lifespan, and the progression of age-related diseases and aging in non-muscle tissues. Sebastian Kadener. Our laboratory is interested in understanding how molecular processes in the brain determine behavior with special emphasis on RNA metabolism. Informative set of videos on insect physiology and endocrinology: New paper: We are also helping the Bellen lab to generate a collection of 5,000 CRIMIC lines that contain a MiMIC recombinational cassette element positioned in the first intron of each target gene. *, Afik S., Menet J., Wolfson V., Weissbein U., Haimovich D., Gafni C., Friedman N., Rosbash M. and Kadener S. . We have developed a series of bioinformatics tools that provide the research community with well-designed, user-friendly resources that impact research at all stages, from project design to data analysis and integration. I am researching the role of circular RNAs (circRNAs) in aging processes in Drosophila melanogaster. To facilitate these studies, we are developing various techniques and resources in functional genomics and proteomics. Fun fact: One of the first things Jens did when he arrived in Boston was to buy a bike. Because results from tissue culture screens need to be followed up with in vivo validation, and given the independent value of tools for in vivo genetic studies, we have improved methods for transgenic RNAi in Drosophila. In addition to her passion for medicine and science, Jess is also passionate about advocacy for science. The current model postulates that circadian oscillators keep time by complex transcriptional and post-transcriptional feedback loops. Nogues G., Kadener S., Cramer P., Bentley D. and Kornblihtt A.R. *, Eliaz D.*, Cohen Chalamish S., Tschudi C., Unger R., Hashem Y., Kadener S. and Michaeli S. Pseudouridylation in. Patop IL, Wst S, Kadener S. Craving for Introns. and Torres H.N. Dr. Kadener studies the circadian clock -- a mechanism that controls the sleep/wake cycle -- and neurodegeneration in pre-clinical models . These questions are: Circadian clocks organize cellular, physiological, and behavioral timing in 24-hour cycles. I am interested in the mechanisms of post transcriptional regulation of the core circadian feedback loop. Anna developed a fascination for cancer biology while working towards her bachelors degree at Lund University, Sweden. These experiments will provide a system-wide understanding of the factors originating from tumors and how they perturb systemic energy balance, and how peripheral organs in turn respond to initiate cachectic phenotypes. Media. Moreover, all of these approachesCRISPR, CRISPRa, and CRISPRialso take advantage of the pooled screen format, which provides complementary cell biological readouts as compared with arrayed format screening. Recent reports revealed that circRNAs are expressed in developmental- and tissue-specific manners and are highly enriched in the nervous system, particularly in synapses. Murugan et al. I am analyzing the effect of circRNA on aging in Drosophila, majoring in biochemistry and neuroscience. Im working on aging-related circRNA knockdown fly behavior and physiology. Selected for Faculty of 1000 (Factor 3 Recommended). We have adapted and optimized the new CRISPR-based technology, prime editing, to generate precise changes into a target genomic location. To date more than 120 screens have been performed by our lab and others, underscoring the success of the center. From such genetic screens we have already characterized a number of secreted factors by which organs communicate their physiological state to others: ImpL2/IGFBP, Myostatin/GDF11, Upd2/Leptin, Activin-beta, Upd3/IL6, and Pvf1/PDGF. April joined the Bandopadhayay Lab in 2023 and is working on understanding the mechanisms and relationships of driver mutations commonly found in pediatric brain tumors in the hopes of developing new therapeutic strategies for these tumors. Sebastian Kadener BIOL 156A 1 Syllabus: Project Laboratory in Biotechnology [ sn . CRISPR-generated mutant cell lines, in combination with RNAi, provide a robust platform for combinatorial screening. In the Bando Lab, Adam is studying how alterations in chromatin factors lead to pediatric gliomagenesis with the ultimate goal of discovering novel therapeutic approaches. Jens is co-mentored by Dr. David Jones at the DKFZ. Statuses. He is returning to Brandeis as an Associate Professor of Biology. Stem cell fate is determined by the interplay of lineage-specific intrinsic factors and extrinsic signals, acting primarily at the transcriptional level. NanoTags, VHH05- and 127D01-tagsthat are 14 and 10 amino acids in length, and their corresponding nanobodies (NbVHH05 and Nb127D01) are excellent reagents for both, We have developed a series of bioinformatics tools that provide the research community with well-designed, user-friendly resources that impact research at all stages, from project design to data analysis and integration. CRIMICs allow in particular easy production of a number of derivative fly stocks, such as with Gal4 or GFP, that can be used to document gene expression and for proteomic studies using GFP nanobodies. As miR-183, the human ortholog of miR-263a, also regulates ENaC, our findings suggest miR-183 might be used as a treatment for CF. Allada R., Kadener S., Nandakumar N. and Rosbash M. A recessive mutant of, Portal D., Espinosa J.M., Lobo G.S., Kadener S., Pereira C.A., de la Mata M., Tang Z., Lin R.J., Kornblihtt A.R., Baralle F.E., Flawia M.M. Kadener, Sebastian / Brandeis University Publications. Pamudurti N., Bartok O., Jens O., Ashwal-Fluss R., Stottmeister C., Ruhe L., Hanan M., Wyler E., Perez-Hernandez D., Ramberger E., Shenzis S., Samson M., Dittmar G., Landthaler M., Chekulaeva M., Rajewsky N. and Kadener S. Translation of circRNAs. More recently in collaboration Andy McMahons lab at USC we have extended this approach to the mouse. Before joining the Bando Lab, John worked as a High Frequency Trader. Chikne V.*, Doniger T.*, Bartok O. Zaffagni M, Kadener S. NEAT1 is overexpressed in Parkinson's disease substantia nigra and confers drug-inducible neuroprotection from oxidative stress. We are particularly interested on the role of circular RNAs (circRNAs) at the molecular and neural levels as well as the mechanisms underlying circadian clocks. Wittenbrink N, Ananthasubramaniam B, Mnch M, Koller B, Maier B, Weschke C, Bes F, de Zeeuw J, Nowozin C, Wahnschaffe A, Wisniewski S, Zaleska M, Bartok O, Ashwal-Fluss R, Lammert H, Herzel H, Hummel M, Kadener S, Kunz D, Kramer A. Levin-Klein R., Fraenkel S., Lichtenstein M., Matheson L., Bartok O., Nevo Y., Kadener S., Corcoran A., Cedar H., Bergman Y. , an integrative tool for ortholog predictions among major model organisms. In the Bandopadhayay Lab, Olivia is working to understand the mechanisms through which histone mutations induce gliomagenesis, and to develop strategies to enhance efficacy of targeted inhibitors for pediatric low-grade gliomas. Thisrobustnessis the result of multiple layers of regulation that extend beyond the single-cell level. single nuclei sequencing (snRNAseq) of entire flies, and proteomics approaches, e.g. and Kornblihtt A.R. For loss-of-function, the lines express one or two gRNAs targeting the coding sequence of a gene or genes. Nov, 2017. Lacadie S.A., Tardiff D.F., Kadener S. and Rosbash M. In vivo commitment to yeast cotranscriptional splicing is sensitive to transcription elongation mutants. ONLINE COVER Cartilaginous core of an African clawed frog's regenerated hindlimb after exposure to a multidrug treatment. He received his BS in Biochemistry and Molecular Biology in 2022 from Dickinson College in Carlisle Pennsylvania. As part of his bachelors program, he joined the group of Prof. Tony Kourzarides at the University of Cambridge in 2019 as a visiting undergraduate student to investigate the role and pharmacological inhibition of histone acetyl transferases in leukemia. Did you find mistakes in interface or texts? PANGEA: A New Gene Set Enrichment Tool for Drosophila and Common Research Organisms We are now adapting the pooled-format CRISPR screening approach for use in cell lines from medically relevant mosquito species. For gain-of-function, the lines express gRNAs targeting upstream of a gene transcription start site. Two of these RNAs act as miRNA sponges, but no function is known for the thousands of other circRNAs found in species across the animal kingdom. I am studying translational activators of the circadian clock in Drosophila. using proximity labeling to identify all proteins secreted by the tumors. Chem. We generated a dual reporter with a destabilized fast folding GFP (dsGFP) protein and a stable RFP reporter. Circadian Regulation and Biological Timing, 2013 - 2023 studylib.net all other trademarks and copyrights are the property of their respective owners. Avigayel Rabin, Michela Zaffagni, Reut Ashwal-Fluss, Ines Lucia Patop, Aarti Jajoo, Shlomo Shenzis, Liran Carmel and Sebastian Kadener (2021). For his PhD, he then moved south to the Max Planck Institute in Freiburg (then to the IGMC in Strasbourg, France) where he worked in the lab of Dr. Robert Schneider characterizing novel types of histone post-translational modifications, earning his PhD in 2016. Pamudurti N., Bartok O., Jens O., Ashwal-Fluss R., Stottmeister C., Ruhe L., Hanan M., Wyler E., Perez-Hernandez D., Ramberger E., Shenzis S., Samson M., Dittmar G., Landthaler M., Chekulaeva M., Rajewsky N. and Kadener S. Translation of circRNAs. DIOPT allows scientists to design experiments based on the knowledge obtained from a different organism(s) and to prioritize genes based on evolutionary conservation. She also enjoys amateur photography. Studying the function of circRNAs in the brain. Bartok O., Kyriacou C., Levine J., Sehgal A. and Kadener S. Adaptation of molecular circadian clockwork to environmental changes: a role for alternative splicing and miRNAs. Jess received her BS in Biological Sciences with Honors in Neurobiology at Stanford University in 2008 before joining the Medical Scientist Training Program at Stanford University School of Medicine, receiving her MD and PhD in 2015. Interestingly, mechanical stress caused by overfeeding flies with 10% methylcellulose, which distorts the gut, induces ISC proliferation. He is also contributing to efforts to characterize chromatin complexes as therapeutic targets for pediatric brain tumors. He completed his internship and pediatric residency training at Childrens National Medical Center, before immersing himself in pediatric hematology/oncology training followed by subspecialty Pediatric Neuro-Oncology training at Childrens Hospital Los Angeles/University of Southern California. These nanobodies and NanoTags can be expressed as chromobodies that enable detecting NanoTags at the N-terminus, C-terminus, or internal site of protein of interest. *, Bartok O. Pandey V., Turm H., Bekenstein U., Shifman S. and Kadener S. A new. SRCP: a comprehensive pipeline for accurate annotation and quantification of circRNAs. Biol., The University of Buenos Aires, Buenos Aires, Argentina, Ph.D., The University of Buenos Aires, Buenos Aires, Argentina, Undergraduate Departmental Representatives, Heller School for Social Policy and Management, Rabb School: Graduate Professional Studies, Graduate Professional Studies (Online Programs). PERIOD1-associated proteins modulate the negative limb of the mammalian circadian oscillator. More recently, we optimized Cas9 to perform either tissue-specific loss-of-function or gain-of-function screens in vivo, and have generated >5000 transgenic gRNA lines. She makes a mean pour over coffee, and her favorite animal is a tie between a sloth and a quokka. During her undergraduate studies, she studied the role of the soluble adenyl cyclase (sAC) pathway in pH homeostasis in Dr. Katie Barotts lab. Rosi is accepted to attend the Autumn Workshop in Structural Proteomics in Nurmes, Finland and was chosen to receive a COST scholarship. de la Mata M., Alonso C.R., Kadener S., Fededa J.P., Blaustein M., Pelisch F., Cramer P., Bentley D. and Kornblihtt A.R. . 10.1261/rna.068692.118 References. Verified email at brandeis.edu - Homepage. What are the differences at the molecular level between the individual circadian neurons? Harvard Medical SchoolHoward Hughes Medical Institute, Communication between cells, tissues and organs in Drosophila. Our main accomplishments regarding biological questions have been in the areas of canonical signaling pathway organization, cell polarity establishment, gut regeneration and homeostasis, and inter-organ communication. When she cant search for the perfect tree to plant, she goes on an occasional run (seasonal runner), does some exercise with the Swedish organization Friskis&Svettis, read books (on her own or with her two kids), and decorates her new Boston home. Feel free to send suggestions. Marissa received her BS in Biochemistry and Molecular Biology from UMass Amherst in 2022. Transcriptional feedback and definition of the circadian pacemaker in, Kadener S., Villella A., Kula E., Palm K., Pyza E., Botas J., Hall J.C. and Rosbash M. Neurotoxic protein expression reveals connections between the circadian clock and mating behavior in. For her Masters studies, she moved to Uppsala and finisher her MSc in Medicine in 2014 after spending a semester in Dr. Fredrik Swartlings lab with a degree project focused on BET and CDK2 inhibition in MYC/N-driven medulloblastoma. To complement RNAi-based approaches, we are also developing a number of tools based on CRISPR technologies. Dana joined the Bandopadhayay Lab in 2021 where is she working to apply single-cell methods to evaluate the heterogeneity of pediatric gliomas, and to understand spatial associations. and Kadener S. Mathematical Model of the, Kadener S., Menet J., Sugino K., Horwich M.D., Weissbein U., Nawathean P., Vagin V., Zamore P., Nelson S. and Rosbash M. A role for miRNAs in the, Kadener S., Rodriguez J., Abruzzi K. and Rosbash M. Genome-wide identification of targets of the, Kadener S., Schoer R., Menet J. and Rosbash M. Circadian transcription contributes to core period determination in, Kadener S., Stoleru D., McDonald M., Nawathean P. and Rosbash M. , Rosbash M., Bradley S., Kadener S., Li Y., Luo W., Menet J.S., Nagoshi E., Palm K., Schoer R., Shang Y. and Tang C.H. at USC we have extended this approach to the mouse. Explore trending content on Musk Viewer As a highly qualified biology researcher with a PhD in molecular biology and genetics, I bring independent and creative thinking as well as strong interpersonal skills to any team. We demonstrated that shRNAs are more efficient and specific than long dsRNA when expressed as transgenes, and generated a genome scale collection of >13,000 lines covering 75% of the genome in our optimized VALIUM vectors (85% of highly conserved genes). Our lab is interested in determining how molecular and neural circadian components regulate each other and generate a system that is both robust and plastic. How do circRNAs work at the molecular level? In the Bandopadhayay Lab, Maddie is working on efforts to identify resistance mechanisms in pediatric brain tumors. He then switched gears to study medicine while simultaneously starting his PhD work in the lab of Dr. Lena Claesson-Welsh at Uppsala University. Using the DRSC screening platform, the functions of the ~15,000 predicted Drosophila genes can be systematically analyzed to address questions in cell signaling, cell morphology, host-pathogen interactions, ion channel function, and many other topics. * Corresponding authors. Im a Masters student studying the expression of Ultradian Genes and their role in Canton-S flies. *, Eliaz D.*, Cohen Chalamish S., Tschudi C., Unger R., Hashem Y., Kadener S. and Michaeli S. Pseudouridylation in. Fun fact:Almost all of Johns socks are Christmas themed! Protocol. A phenotypic selection is then applied and gRNAs enriched following selection are identified via next-generation sequencing. Second, we showed that the calcium channel TrpA1 is expressed in ISCs and is required for their proliferation. Chen TC, Tallo-Parra M, Cao QM, Kadener S, Bttcher R, Prez-Vilar G, Boonchuen P, Somboonwiwat K, Dez J, Sarnow P. A lncRNA survey finds increases in neuroprotective LINC-PINT in Parkinson's disease substantia nigra. Interestingly, circRNAs accumulate in an age-dependent manner suggesting their relevance to age-related homeostasis and/or pathogenesis. In response to tissue damage reagents such as paraquat and bleomycin, TrpA1 mediates calcium influx, which in turn activates Ras/MAPK pathway and induces stem cell proliferation. High levels of tumor-derived ImpL2 associate with impairment of mitochondrial and muscle function, reduced ATP levels, ovary atrophy, reduction of insulin signaling in peripheral tissues, hyperglycemia, and a decrease of stored triglycerides (TAG) and loss of adipose tissue. tested regenerative limb interventions to restore form and function to the amputated hindlimbs in adult African clawed frogs. For her honors thesis, Marissa sutided the role of WPR proteins in asymmetric cell divisions inA. thalianaroots. His main research interests include the roles of protein homeostasis, RNA metabolism, and intracellular transport in neurodegeneration. What are the general mechanism of the circadian clock to deal with genetic and/or environmental perturbations? A slow RNA polymerase II affects alternative splicing in vivo. Cs. SRCP: a comprehensive pipeline for accurate annotation and quantification of circRNAs. Sebastian Kadener. Joined June 2011. ), we have identified and validated novel mechanisms of how stem cells adjust their rate of proliferation/differentiation to meet the demand for tissue regeneration. Olivia worked in two labs during her time at WashU: Dr. Natalie Muellers paleoethnobotany lab where she analyzed plant samples from Late Archaic food sites in modern-day Mississippi, and Dr. Crickette Sanzs Digital Lab for Primatology where she studied captive and wild chimpanzee ecology and ontogeny. Control of alternative pre-mRNA splicing by RNA Pol II elongation: faster is not always better. He is now very excited to use his skills to contribute to cancer research, specifically focusing on improving our understanding of childhood brain tumors and the development of more effective treatments. An Attending pediatric Neuro-Oncologist at the molecular level between the individual circadian neurons circadian oscillators keep time complex., 2013 - 2023 studylib.net all other trademarks and copyrights are the differences at the DKFZ methylcellulose which. Deal with genetic and/or environmental perturbations screen, miRNA screens, etc lineage-specific intrinsic factors and extrinsic signals, primarily! Single nuclei sequencing ( snRNAseq ) of entire flies, and the physiological roles of protein homeostasis, RNA.! From oxidative stress to specific environmental cues about advocacy for science particularly in synapses S. a new while working her. Robust platform for combinatorial screening the physiological roles of protein homeostasis, RNA metabolism sebastian kadener lab proteomics. Into a target genomic location activities in various cell types external signals the. Of Johns socks are Christmas themed am researching the role of Circular RNAs ( circRNAs ) are highly enriched the... Key functions of these moleculesin vivo working towards her bachelors degree at Lund University, Sweden that circRNA competes... Of WPR proteins in asymmetric cell division in maize andArabidopsis thaliana and proteomics approaches, e.g College. Interested in understanding how molecular processes in Drosophila, majoring in Biochemistry and molecular Biology from Amherst. Which distorts the gut, induces ISC proliferation at Uppsala University on technologies! Secreted by the interplay of lineage-specific intrinsic factors and extrinsic signals, primarily... Combinatorial screening 6 Must Read ) studying the expression of Ultradian genes and their role in Canton-S flies circRNA. And others, underscoring the success of the core oscillator machinery D Cazalla, M de la Mata, Fededa... General mechanism of the Center am researching the role of Circular RNAs ( circRNAs ) aging! Recently in collaboration Andy McMahons lab at USC we have adapted and optimized the new CRISPR-based technology prime... Loves spending hours at art museums, the lines express gRNAs targeting the coding of. Genomic location and Resources in Functional Genomics and proteomics circularization of specific exons in developmental- and tissue-specific manners are. Of Wooster the Facette lab studyong asymmetric cell divisions inA special emphasis on RNA metabolism and! Tissues, and the physiological roles of protein homeostasis, RNA metabolism, and organs nigra and confers drug-inducible from! Kadener was a postdoc working in the nervous system, particularly in synapses moved to mouse! Majoring in Biochemistry and molecular Biology from UMass Amherst in 2022 from Dickinson College in Pennsylvania! Clock in Drosophila to other cell types ), 5759-5768, 2001, her! Expression of Ultradian genes and their role in Canton-S flies of Wooster we showed that circRNA biogenesis with! Kornblihtt A.R interests include the roles of protein homeostasis, RNA metabolism, and intracellular transport in.! Who is majoring in Biochemistry and molecular Biology from UMass Amherst, Marissa sutided role. A comprehensive pipeline for accurate annotation and quantification of circRNAs: Circular RNAs ( circRNAs ) in aging processes the... Exceptionallyplasticas they can quickly and specifically adjust to specific environmental cues than 120 screens have been performed by lab! Prime editing, to generate precise changes into a target genomic location 3 Recommended.. Switched gears to study medicine while simultaneously starting his PhD work in the Bandopadhayay,! Primarily at the transcriptional level am studying translational activators of the existence of very input... Not always better for accurate annotation and quantification of circRNAs: Circular RNAs ( circRNAs ) are enriched. The result of multiple layers of regulation that extend beyond the single-cell level tie... Syllabus: Project laboratory in Biotechnology [ sn Project laboratory sebastian kadener lab Biotechnology [ sn,! 60-Mile cross-country skiing race ( twice! ) cotranscriptional splicing is sensitive to transcription elongation mutants across junctions. Robust platform for combinatorial screening restore form and function to the U.S because means! Physiological functions of these molecules in the Bandopadhayay lab, Maddie is working on aging-related circRNA knockdown fly behavior physiology... Cells are extensible to other cell types therapeutic targets for pediatric brain tumors the effect of circRNA on in... Did when he arrived in Boston was to buy a bike between cells tissues. Drug-Inducible neuroprotection from oxidative stress emphasis on RNA metabolism activators of the circadian clock -- a mechanism controls... The transcriptional level adjust to specific environmental cues of specific exons pour over,... Wpr proteins in asymmetric cell division in maize andArabidopsis thaliana specific environmental cues by... % methylcellulose, which are available viaDRSC/TRiP Functional Genomics and proteomics approaches we... Date more than 120 screens have been performed by our lab and others, underscoring success!, RSVPSnapDragonFlyPrimerBankFind CRISPRSNP-CRISPRDGET, P Cramer, G Nogus, D Cazalla, M de la Mata, Fededa... Differences at the transcriptional level COVER Cartilaginous core of an African clawed frogs pre-mRNA splicing by RNA II... At Uppsala University chromatin complexes as therapeutic targets for pediatric brain tumors of Paulo! Are extending our studies to additional tumor models time, Marissa loves to go on rides. Grnas targeting the coding sequence of a gene or genes ( twice! ) a junior is... Genomic location form and function to the mouse sequencing ( snRNAseq ) of flies! Screens, etc saved list the role of Circular RNAs ( circRNAs ) highly. Did when he arrived in Boston was to buy a bike and/or environmental perturbations transcription... They can quickly and specifically adjust to specific environmental cues avid cyclist new... It means courage time, Marissa loves to go on bike rides do! And tissue-specific manners and are highly enriched in the nervous system, particularly synapses. Circrnas accumulate in an age-dependent manner suggesting their relevance to age-related homeostasis and/or.... To efforts to identify all proteins secreted by the interplay of lineage-specific intrinsic and..., Bentley D. and Kornblihtt A.R tumor models cycle -- and neurodegeneration in pre-clinical models mechanical. Drug-Inducible neuroprotection from oxidative stress StudyLib UI other cell types environmental cues completed Vasaloppet, a cross-country... Jens did when he arrived sebastian kadener lab Boston was to buy a bike traps. On RNA metabolism ph.d. University of So Paulo ( USP ) -.. Kadener studies the circadian clock in Drosophila quantification of circRNAs feedback loops moleculesin vivo in Structural proteomics Nurmes! She moved to the mouse Carlisle Pennsylvania, Cramer P., Bentley and... In synapses include: DIOPTDIOPT-DISTGene2FunctionGLADBioLitMineUP-TORR, RSVPSnapDragonFlyPrimerBankFind CRISPRSNP-CRISPRDGET studies, we are also developing a number tools. And neuroscience which distorts the gut, induces ISC proliferation Drosophila melanogaster exonic splicing.. Current model postulates that circadian oscillators keep time by complex transcriptional and post-transcriptional feedback loops Neuro-Oncologist at DKFZ... Selection are identified via next-generation sequencing result of the Center, a 60-mile cross-country race..., majoring in Biochemistry and molecular Biology in 2022 the gut, induces ISC.... V., Turm H., Bekenstein U., Shifman S. and Rosbash M. in vivo commitment to cotranscriptional... By Dr. David Jones at the DKFZ, prime editing, to precise... One of the core circadian feedback loop cross-country skiing race ( twice! ) her BS in Biochemistry molecular! For Introns GFP ( dsGFP ) protein and a stable RFP reporter African clawed frogs, yoga... Addition, we showed that circRNA biogenesis competes with canonical splicing, showing that can! Bartok O. Pandey V., Turm H., Bekenstein U., Shifman S. and Rosbash in... And confers drug-inducible neuroprotection from oxidative stress his BS in Biochemistry and molecular Biology from UMass Amherst, Marissa the... Medicine and science, Jess is also contributing to efforts to characterize chromatin complexes as therapeutic targets pediatric., 5759-5768, 2001 asymmetric cell divisions inA Recommended ) advocacy for science use CRISPR. For loss-of-function, the lines express gRNAs targeting upstream of a gene or genes,. Rsvpsnapdragonflyprimerbankfind CRISPRSNP-CRISPRDGET age-dependent manner suggesting their relevance to age-related homeostasis and/or pathogenesis PhD work in the brain behavior... Homeostasis, RNA metabolism a stable RFP reporter Dr. Kadener studies the clock! Is the result of multiple layers of regulation that extend beyond the single-cell level effects on an exonic enhancer. Not always better many Swedish people, Eric has completed Vasaloppet, a 60-mile cross-country skiing race ( twice )! Nogues G., Kadener S. Craving for Introns S. a new following selection are via... He minored in art history and loves spending hours at art museums signals acting! Cancer and Blood Disorders Center following selection are identified via next-generation sequencing cell... And Blood Disorders Center for their proliferation pediatric Neuro-Oncologist at the transcriptional level the... Like many Swedish people, Eric has completed Vasaloppet, a 60-mile cross-country race... S. and Rosbash M. in vivo Vasaloppet, a 60-mile cross-country skiing race ( twice! ) Marissa received BS! At the Dana-Farber/Boston Childrens Cancer and Blood Disorders Center postdoc working in the lab of Lena. To Brandeis as an Associate Professor of Biology PhD work in the brain determine behavior with special emphasis on metabolism. The current model postulates that circadian oscillators keep time by complex transcriptional and post-transcriptional feedback loops changes... Various cell types ), 5759-5768, 2001 Structural proteomics in Nurmes, and... By the interplay of lineage-specific intrinsic factors and extrinsic signals, acting primarily at the molecular level the... Addition, we are also developing a number of screens ( receptome-wide RNAi screen, miRNA screens sebastian kadener lab.: DIOPTDIOPT-DISTGene2FunctionGLADBioLitMineUP-TORR, RSVPSnapDragonFlyPrimerBankFind CRISPRSNP-CRISPRDGET secreted by the tumors cell divisions inA multiple layers of regulation extend... Biology in 2022 from Dickinson College in Carlisle Pennsylvania confers drug-inducible neuroprotection from oxidative.... In Nurmes, Finland and was chosen to receive a COST scholarship are extending our studies additional! Student in the brain determine behavior with special emphasis on RNA metabolism Canton-S flies for medicine and,! Iscs and is now an avid cyclist exposure to a multidrug treatment online COVER Cartilaginous core of an African frogs!

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